HPLC Column, NP Amino, 3um, 2.1mm ID x 150mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino™ is a normal-phase and HILIC stationary phase designed for the retention and separation of highly polar compounds. Manufactured using a three-carbon propyl ligand with a terminal amino functional group bonded to high-purity silica, this stationary phase provides selectivity that is particularly useful for carbohydrates, organic acids, and other polar analytes. As a USP L8 equivalent phase, it is widely used as an alternative in established USP methods and for new method development involving Normal Phase and HILIC chromatography.
68340-15P-2 is an HPLC column packed with 3 µm NP Amino™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. This narrow-bore configuration combines high chromatographic resolution with reduced solvent consumption and compatibility with LC-MS instrumentation. The 150 mm length provides increased peak capacity and resolving power compared to shorter column formats, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.2–0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of precise and reproducible low-flow operation.
This column is particularly useful for the analysis of sugars, carbohydrates, compounds containing carboxylic acid groups, and other polar molecules. Suitable for both Normal Phase and HILIC chromatography, the amino functionality provides retention characteristics that make it valuable for analytes that are poorly retained on conventional reversed-phase columns. The 2.1 mm ID format is commonly used for LC-MS applications requiring excellent sensitivity and low solvent consumption. As an unmodified silica bonded amino phase, this column is also suitable for HILIC applications.
Key Benefits
NP Amino™ stationary phase
USP L8 equivalent chemistry
Amino bonded phase
High-purity silica support
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced retention of polar compounds
3 µm particle size
Greater efficiency than 5 µm columns
Excellent for LC-MS applications
Typical Applications
USP L8 methods
Sugar analysis
Carbohydrate analysis
Organic acid analysis
Polar compound analysis
HILIC methods
Normal Phase chromatography
Food and beverage analysis
Pharmaceutical analysis
LC-MS methods
Specifications
Product Type: HPLC Column
Stationary Phase: NP Amino™
USP Classification: L8
Chromatography Modes: Normal Phase and HILIC
Bonded Phase: Amino
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 100 Å
Column Dimensions: 2.1 mm ID x 150 mm
Length: 150 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for highly polar compounds
Commonly used for sugars and compounds containing carboxylic acid groups
Suitable for both Normal Phase and HILIC chromatography
As an unmodified silica bonded amino phase, this column is suitable for HILIC applications
The 2.1 mm ID format is ideal for reduced solvent consumption and LC-MS compatibility
Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
The 150 mm length provides greater resolution and peak capacity than shorter column formats
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Packed and shipped in Normal Phase solvents